Journal: International journal of molecular sciences

Article Title: Effectiveness of Combinational Treatments for Alzheimer's Disease with Human Neural Stem Cells and Microglial Cells Over-Expressing Functional Genes.

doi: 10.3390/ijms24119561

Figure Lengend Snippet: Figure 1. Expression of mRNAs and production of functional proteins in human neural stem cells and microglial cells, and amyloid-β (Aβ) clearance by microglial cells. (A) RT-PCR analysis of ChAT, NEP and SRA mRNAs. (B) Immunocytochemical staining on ChAT, NEP and SRA (green). (C) Clearance of Aβ peptides by HMO6 (#), HMO6.NEP (•) and HMO6.SRA (▼). * Significantly different from HMO6 cells at each time point (p < 0.05).

Article Snippet: Immunohistochemical Analysis of hMito and Functional Proteins The mouse brains were perfusion-fixed with 4% paraformaldehyde solution and postfixed for 48 h, followed by cryoprotection in 30% sucrose for 72 h. Coronal cryosections in 30-μm thickness were prepared and processed for double immunostaining of human mitochondria (hMito) and/or ChAT, NEP or SRA using antibodies specific for hMito (1:100, Millipore), ChAT (1:100, Invitrogen), NEP (1:100, Invitrogen) and SRA (1:100, Bioss).

Techniques: Expressing, Functional Assay, Reverse Transcription Polymerase Chain Reaction, Staining

Journal: International journal of molecular sciences

Article Title: Effectiveness of Combinational Treatments for Alzheimer's Disease with Human Neural Stem Cells and Microglial Cells Over-Expressing Functional Genes.

doi: 10.3390/ijms24119561

Figure Lengend Snippet: Figure 4. Recovery of learning and memory functions four weeks post-injection in passive avoid- ance (A) Morris water-maze (B) studies, and tracking of water-maze swimming performance (C). #: Normal, •: AF64A alone, ▼: F3, △: F3.ChAT, ■: HMO6, □: HMO6.NEP, ◆: HMO6.SRA, 3: F3.ChAT + HMO6.NEP, ▲: F3.ChAT + HMO6.SRA. * Significantly different from normal (p < 0.05). # Significantly different from AF64A alone (p < 0.05).

Article Snippet: Immunohistochemical Analysis of hMito and Functional Proteins The mouse brains were perfusion-fixed with 4% paraformaldehyde solution and postfixed for 48 h, followed by cryoprotection in 30% sucrose for 72 h. Coronal cryosections in 30-μm thickness were prepared and processed for double immunostaining of human mitochondria (hMito) and/or ChAT, NEP or SRA using antibodies specific for hMito (1:100, Millipore), ChAT (1:100, Invitrogen), NEP (1:100, Invitrogen) and SRA (1:100, Bioss).

Techniques: Injection

Journal: International journal of molecular sciences

Article Title: Effectiveness of Combinational Treatments for Alzheimer's Disease with Human Neural Stem Cells and Microglial Cells Over-Expressing Functional Genes.

doi: 10.3390/ijms24119561

Figure Lengend Snippet: Figure 5. Immunohistochemical identification of human neural stem cells (F3) and microglial cells (HMO6) and functional proteins (ChAT, NEP and SRA) via double immunostaining for human mitochondria (hMito) and ChAT, NEP or SRA.

Article Snippet: Immunohistochemical Analysis of hMito and Functional Proteins The mouse brains were perfusion-fixed with 4% paraformaldehyde solution and postfixed for 48 h, followed by cryoprotection in 30% sucrose for 72 h. Coronal cryosections in 30-μm thickness were prepared and processed for double immunostaining of human mitochondria (hMito) and/or ChAT, NEP or SRA using antibodies specific for hMito (1:100, Millipore), ChAT (1:100, Invitrogen), NEP (1:100, Invitrogen) and SRA (1:100, Bioss).

Techniques: Immunohistochemical staining, Functional Assay, Double Immunostaining